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n terminal mgfp vector  (OriGene)


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    OriGene n terminal mgfp vector
    Identification of a <t>modular</t> <t>PRR14</t> nuclear lamina binding domain. (A) Representative confocal imaging of HeLa cells showing localization of H3K9me3-marked heterochromatin (cyan), GFP-tagged full-length PRR14 (green), and Lamin A/C (red). (B) A schematic model of H3K9me3/HP1 heterochromatin tethering to the nuclear lamina via PRR14. See color scheme in panel C. (C) A schematic representation of the proposed PRR14 modular domain organization. Domains, LAVVL motif and nuclear localization sequences (NLS) are indicated. (D) Representative confocal images of live HeLa cells expressing mCherry–Lamin A transfected with GFP-tagged PRR14 fragments, as indicated (wild type, WT). The <t>N-terminal</t> fragment (1–135) shows localization to heterochromatin, the centrally located (231–282) fragment includes a surrogate SV40 NLS and is identified as a minimal nuclear lamina binding domain (LBD), the Tantalus domain (455–517) fragment localizes throughout the nucleoplasm and cytoplasm and the C-terminal fragment (366–585) localizes in the nucleoplasm. Scale bars: 5 µm. Images in A and D are representative of three experiments and >60 cells.
    N Terminal Mgfp Vector, supplied by OriGene, used in various techniques. Bioz Stars score: 91/100, based on 18 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/n+terminal+mgfp+vector/pCMV6-AN-mGFP+Mammalian+Expression+Vector/pmc07272351-289-8-12
    Average 91 stars, based on 18 article reviews
    n terminal mgfp vector - by Bioz Stars, 2026-09
    91/100 stars

    Images

    1) Product Images from "The PRR14 heterochromatin tether encodes modular domains that mediate and regulate nuclear lamina targeting"

    Article Title: The PRR14 heterochromatin tether encodes modular domains that mediate and regulate nuclear lamina targeting

    Journal: Journal of Cell Science

    doi: 10.1242/jcs.240416

    Identification of a modular PRR14 nuclear lamina binding domain. (A) Representative confocal imaging of HeLa cells showing localization of H3K9me3-marked heterochromatin (cyan), GFP-tagged full-length PRR14 (green), and Lamin A/C (red). (B) A schematic model of H3K9me3/HP1 heterochromatin tethering to the nuclear lamina via PRR14. See color scheme in panel C. (C) A schematic representation of the proposed PRR14 modular domain organization. Domains, LAVVL motif and nuclear localization sequences (NLS) are indicated. (D) Representative confocal images of live HeLa cells expressing mCherry–Lamin A transfected with GFP-tagged PRR14 fragments, as indicated (wild type, WT). The N-terminal fragment (1–135) shows localization to heterochromatin, the centrally located (231–282) fragment includes a surrogate SV40 NLS and is identified as a minimal nuclear lamina binding domain (LBD), the Tantalus domain (455–517) fragment localizes throughout the nucleoplasm and cytoplasm and the C-terminal fragment (366–585) localizes in the nucleoplasm. Scale bars: 5 µm. Images in A and D are representative of three experiments and >60 cells.
    Figure Legend Snippet: Identification of a modular PRR14 nuclear lamina binding domain. (A) Representative confocal imaging of HeLa cells showing localization of H3K9me3-marked heterochromatin (cyan), GFP-tagged full-length PRR14 (green), and Lamin A/C (red). (B) A schematic model of H3K9me3/HP1 heterochromatin tethering to the nuclear lamina via PRR14. See color scheme in panel C. (C) A schematic representation of the proposed PRR14 modular domain organization. Domains, LAVVL motif and nuclear localization sequences (NLS) are indicated. (D) Representative confocal images of live HeLa cells expressing mCherry–Lamin A transfected with GFP-tagged PRR14 fragments, as indicated (wild type, WT). The N-terminal fragment (1–135) shows localization to heterochromatin, the centrally located (231–282) fragment includes a surrogate SV40 NLS and is identified as a minimal nuclear lamina binding domain (LBD), the Tantalus domain (455–517) fragment localizes throughout the nucleoplasm and cytoplasm and the C-terminal fragment (366–585) localizes in the nucleoplasm. Scale bars: 5 µm. Images in A and D are representative of three experiments and >60 cells.

    Techniques Used: Binding Assay, Imaging, Expressing, Transfection

    Related Articles

    Plasmid Preparation:

    Article Title: The PRR14 heterochromatin tether encodes modular domains that mediate and regulate nuclear lamina targeting
    Article Snippet: The mouse PRR14 expression vector was obtained from Origene Technologies, encoding a C-terminal fusion with TurboGFP (cat# MG209414).The mouse PRR14 expression vector was obtained from Origene Technologies, encoding a C-terminal fusion with TurboGFP (cat# MG209414).. The mouse PRR14 ORF was transferred to the N-terminal mGFP vector from OriGene (pCMV6-AN-mGFP, cat# PS100048) using the Origene PrecisionShuttle system.. For analyses of candidate modular LBDs from human, Xenopus , and gecko PRR14, and for expression of the isolated Tantalus domain, gene synthesis was used (Genewiz).For analyses of candidate modular LBDs from human, Xenopus , and gecko PRR14, and for expression of the isolated Tantalus domain, gene synthesis was used (Genewiz).

    Article Title: The PRR14 heterochromatin tether encodes modular domains that mediate and regulate nuclear lamina targeting
    Article Snippet: mouse PRR14 expression vector was obtained from Origene Technologies, Inc., encoding a C-terminal fusion with TurboGFP (cat# MG209414). .. The mouse PRR14 orf was transferred to the N-terminal mGFP vector from OriGene (pCMV6-AN-mGFP, cat# PS100048) using the Origene PrecisionShuttle system. .. For analyses of candidate modular LBDs from human, xenopus, and gecko PRR14, and for expression of the isolated Tantalus domain, gene synth

    Article Title: The Human Protein PRR14 Tethers Heterochromatin to the Nuclear Lamina During Interphase and Mitotic Exit
    Article Snippet: expression vectors were obtained from OriGene Technologies, Inc., encoding C-terminal fusions with TurboGFP (RG208696) or a Myc-DDK-tag (RC208696). .. The PRR14 orf was also transferred to an N-terminal mGFP vector from OriGene (PS100048). .. Deletion mutagenesis was carried out using restriction enzyme sites within the PRR14 orf.

    Article Title: The Human Protein PRR14 Tethers Heterochromatin to the Nuclear Lamina During Interphase and Mitotic Exit
    Article Snippet: Two PRR14 expression vectors were obtained from OriGene Technologies, Inc., encoding C-terminal fusions with TurboGFP (RG208696) or a Myc-DDK-tag (RC208696).Two PRR14 expression vectors were obtained from OriGene Technologies, Inc., encoding C-terminal fusions with TurboGFP (RG208696) or a Myc-DDK-tag (RC208696).. The PRR14 orf was also transferred to an N-terminal mGFP vector from OriGene (PS100048).. Deletion mutagenesis was carried out using restriction enzyme sites within the PRR14 orf.Deletion mutagenesis was carried out using restriction enzyme sites within the PRR14 orf.

    Binding Assay:

    Article Title: The PRR14 heterochromatin tether encodes modular domains that mediate and regulate nuclear lamina targeting
    Article Snippet: The mouse PRR14 expression vector was obtained from Origene Technologies, encoding a C-terminal fusion with TurboGFP (cat# MG209414).The mouse PRR14 expression vector was obtained from Origene Technologies, encoding a C-terminal fusion with TurboGFP (cat# MG209414).. The mouse PRR14 ORF was transferred to the N-terminal mGFP vector from OriGene (pCMV6-AN-mGFP, cat# PS100048) using the Origene PrecisionShuttle system.. For analyses of candidate modular LBDs from human, Xenopus , and gecko PRR14, and for expression of the isolated Tantalus domain, gene synthesis was used (Genewiz).For analyses of candidate modular LBDs from human, Xenopus , and gecko PRR14, and for expression of the isolated Tantalus domain, gene synthesis was used (Genewiz).

    Article Title: The PRR14 heterochromatin tether encodes modular domains that mediate and regulate nuclear lamina targeting
    Article Snippet: mouse PRR14 expression vector was obtained from Origene Technologies, Inc., encoding a C-terminal fusion with TurboGFP (cat# MG209414). .. The mouse PRR14 orf was transferred to the N-terminal mGFP vector from OriGene (pCMV6-AN-mGFP, cat# PS100048) using the Origene PrecisionShuttle system. .. For analyses of candidate modular LBDs from human, xenopus, and gecko PRR14, and for expression of the isolated Tantalus domain, gene synth

    Article Title: The Human Protein PRR14 Tethers Heterochromatin to the Nuclear Lamina During Interphase and Mitotic Exit
    Article Snippet: expression vectors were obtained from OriGene Technologies, Inc., encoding C-terminal fusions with TurboGFP (RG208696) or a Myc-DDK-tag (RC208696). .. The PRR14 orf was also transferred to an N-terminal mGFP vector from OriGene (PS100048). .. Deletion mutagenesis was carried out using restriction enzyme sites within the PRR14 orf.

    Article Title: The Human Protein PRR14 Tethers Heterochromatin to the Nuclear Lamina During Interphase and Mitotic Exit
    Article Snippet: Two PRR14 expression vectors were obtained from OriGene Technologies, Inc., encoding C-terminal fusions with TurboGFP (RG208696) or a Myc-DDK-tag (RC208696).Two PRR14 expression vectors were obtained from OriGene Technologies, Inc., encoding C-terminal fusions with TurboGFP (RG208696) or a Myc-DDK-tag (RC208696).. The PRR14 orf was also transferred to an N-terminal mGFP vector from OriGene (PS100048).. Deletion mutagenesis was carried out using restriction enzyme sites within the PRR14 orf.Deletion mutagenesis was carried out using restriction enzyme sites within the PRR14 orf.

    Imaging:

    Article Title: The PRR14 heterochromatin tether encodes modular domains that mediate and regulate nuclear lamina targeting
    Article Snippet: The mouse PRR14 expression vector was obtained from Origene Technologies, encoding a C-terminal fusion with TurboGFP (cat# MG209414).The mouse PRR14 expression vector was obtained from Origene Technologies, encoding a C-terminal fusion with TurboGFP (cat# MG209414).. The mouse PRR14 ORF was transferred to the N-terminal mGFP vector from OriGene (pCMV6-AN-mGFP, cat# PS100048) using the Origene PrecisionShuttle system.. For analyses of candidate modular LBDs from human, Xenopus , and gecko PRR14, and for expression of the isolated Tantalus domain, gene synthesis was used (Genewiz).For analyses of candidate modular LBDs from human, Xenopus , and gecko PRR14, and for expression of the isolated Tantalus domain, gene synthesis was used (Genewiz).

    Article Title: The PRR14 heterochromatin tether encodes modular domains that mediate and regulate nuclear lamina targeting
    Article Snippet: mouse PRR14 expression vector was obtained from Origene Technologies, Inc., encoding a C-terminal fusion with TurboGFP (cat# MG209414). .. The mouse PRR14 orf was transferred to the N-terminal mGFP vector from OriGene (pCMV6-AN-mGFP, cat# PS100048) using the Origene PrecisionShuttle system. .. For analyses of candidate modular LBDs from human, xenopus, and gecko PRR14, and for expression of the isolated Tantalus domain, gene synth

    Article Title: The Human Protein PRR14 Tethers Heterochromatin to the Nuclear Lamina During Interphase and Mitotic Exit
    Article Snippet: expression vectors were obtained from OriGene Technologies, Inc., encoding C-terminal fusions with TurboGFP (RG208696) or a Myc-DDK-tag (RC208696). .. The PRR14 orf was also transferred to an N-terminal mGFP vector from OriGene (PS100048). .. Deletion mutagenesis was carried out using restriction enzyme sites within the PRR14 orf.

    Article Title: The Human Protein PRR14 Tethers Heterochromatin to the Nuclear Lamina During Interphase and Mitotic Exit
    Article Snippet: Two PRR14 expression vectors were obtained from OriGene Technologies, Inc., encoding C-terminal fusions with TurboGFP (RG208696) or a Myc-DDK-tag (RC208696).Two PRR14 expression vectors were obtained from OriGene Technologies, Inc., encoding C-terminal fusions with TurboGFP (RG208696) or a Myc-DDK-tag (RC208696).. The PRR14 orf was also transferred to an N-terminal mGFP vector from OriGene (PS100048).. Deletion mutagenesis was carried out using restriction enzyme sites within the PRR14 orf.Deletion mutagenesis was carried out using restriction enzyme sites within the PRR14 orf.

    Expressing:

    Article Title: The PRR14 heterochromatin tether encodes modular domains that mediate and regulate nuclear lamina targeting
    Article Snippet: The mouse PRR14 expression vector was obtained from Origene Technologies, encoding a C-terminal fusion with TurboGFP (cat# MG209414).The mouse PRR14 expression vector was obtained from Origene Technologies, encoding a C-terminal fusion with TurboGFP (cat# MG209414).. The mouse PRR14 ORF was transferred to the N-terminal mGFP vector from OriGene (pCMV6-AN-mGFP, cat# PS100048) using the Origene PrecisionShuttle system.. For analyses of candidate modular LBDs from human, Xenopus , and gecko PRR14, and for expression of the isolated Tantalus domain, gene synthesis was used (Genewiz).For analyses of candidate modular LBDs from human, Xenopus , and gecko PRR14, and for expression of the isolated Tantalus domain, gene synthesis was used (Genewiz).

    Article Title: The PRR14 heterochromatin tether encodes modular domains that mediate and regulate nuclear lamina targeting
    Article Snippet: mouse PRR14 expression vector was obtained from Origene Technologies, Inc., encoding a C-terminal fusion with TurboGFP (cat# MG209414). .. The mouse PRR14 orf was transferred to the N-terminal mGFP vector from OriGene (pCMV6-AN-mGFP, cat# PS100048) using the Origene PrecisionShuttle system. .. For analyses of candidate modular LBDs from human, xenopus, and gecko PRR14, and for expression of the isolated Tantalus domain, gene synth

    Article Title: The Human Protein PRR14 Tethers Heterochromatin to the Nuclear Lamina During Interphase and Mitotic Exit
    Article Snippet: expression vectors were obtained from OriGene Technologies, Inc., encoding C-terminal fusions with TurboGFP (RG208696) or a Myc-DDK-tag (RC208696). .. The PRR14 orf was also transferred to an N-terminal mGFP vector from OriGene (PS100048). .. Deletion mutagenesis was carried out using restriction enzyme sites within the PRR14 orf.

    Article Title: The Human Protein PRR14 Tethers Heterochromatin to the Nuclear Lamina During Interphase and Mitotic Exit
    Article Snippet: Two PRR14 expression vectors were obtained from OriGene Technologies, Inc., encoding C-terminal fusions with TurboGFP (RG208696) or a Myc-DDK-tag (RC208696).Two PRR14 expression vectors were obtained from OriGene Technologies, Inc., encoding C-terminal fusions with TurboGFP (RG208696) or a Myc-DDK-tag (RC208696).. The PRR14 orf was also transferred to an N-terminal mGFP vector from OriGene (PS100048).. Deletion mutagenesis was carried out using restriction enzyme sites within the PRR14 orf.Deletion mutagenesis was carried out using restriction enzyme sites within the PRR14 orf.

    Transfection:

    Article Title: The PRR14 heterochromatin tether encodes modular domains that mediate and regulate nuclear lamina targeting
    Article Snippet: The mouse PRR14 expression vector was obtained from Origene Technologies, encoding a C-terminal fusion with TurboGFP (cat# MG209414).The mouse PRR14 expression vector was obtained from Origene Technologies, encoding a C-terminal fusion with TurboGFP (cat# MG209414).. The mouse PRR14 ORF was transferred to the N-terminal mGFP vector from OriGene (pCMV6-AN-mGFP, cat# PS100048) using the Origene PrecisionShuttle system.. For analyses of candidate modular LBDs from human, Xenopus , and gecko PRR14, and for expression of the isolated Tantalus domain, gene synthesis was used (Genewiz).For analyses of candidate modular LBDs from human, Xenopus , and gecko PRR14, and for expression of the isolated Tantalus domain, gene synthesis was used (Genewiz).

    Article Title: The PRR14 heterochromatin tether encodes modular domains that mediate and regulate nuclear lamina targeting
    Article Snippet: mouse PRR14 expression vector was obtained from Origene Technologies, Inc., encoding a C-terminal fusion with TurboGFP (cat# MG209414). .. The mouse PRR14 orf was transferred to the N-terminal mGFP vector from OriGene (pCMV6-AN-mGFP, cat# PS100048) using the Origene PrecisionShuttle system. .. For analyses of candidate modular LBDs from human, xenopus, and gecko PRR14, and for expression of the isolated Tantalus domain, gene synth

    Article Title: The Human Protein PRR14 Tethers Heterochromatin to the Nuclear Lamina During Interphase and Mitotic Exit
    Article Snippet: expression vectors were obtained from OriGene Technologies, Inc., encoding C-terminal fusions with TurboGFP (RG208696) or a Myc-DDK-tag (RC208696). .. The PRR14 orf was also transferred to an N-terminal mGFP vector from OriGene (PS100048). .. Deletion mutagenesis was carried out using restriction enzyme sites within the PRR14 orf.

    Article Title: The Human Protein PRR14 Tethers Heterochromatin to the Nuclear Lamina During Interphase and Mitotic Exit
    Article Snippet: Two PRR14 expression vectors were obtained from OriGene Technologies, Inc., encoding C-terminal fusions with TurboGFP (RG208696) or a Myc-DDK-tag (RC208696).Two PRR14 expression vectors were obtained from OriGene Technologies, Inc., encoding C-terminal fusions with TurboGFP (RG208696) or a Myc-DDK-tag (RC208696).. The PRR14 orf was also transferred to an N-terminal mGFP vector from OriGene (PS100048).. Deletion mutagenesis was carried out using restriction enzyme sites within the PRR14 orf.Deletion mutagenesis was carried out using restriction enzyme sites within the PRR14 orf.

    Stable Transfection:

    Article Title: The PRR14 heterochromatin tether encodes modular domains that mediate and regulate nuclear lamina targeting
    Article Snippet: The mouse PRR14 expression vector was obtained from Origene Technologies, encoding a C-terminal fusion with TurboGFP (cat# MG209414).The mouse PRR14 expression vector was obtained from Origene Technologies, encoding a C-terminal fusion with TurboGFP (cat# MG209414).. The mouse PRR14 ORF was transferred to the N-terminal mGFP vector from OriGene (pCMV6-AN-mGFP, cat# PS100048) using the Origene PrecisionShuttle system.. For analyses of candidate modular LBDs from human, Xenopus , and gecko PRR14, and for expression of the isolated Tantalus domain, gene synthesis was used (Genewiz).For analyses of candidate modular LBDs from human, Xenopus , and gecko PRR14, and for expression of the isolated Tantalus domain, gene synthesis was used (Genewiz).

    Article Title: The PRR14 heterochromatin tether encodes modular domains that mediate and regulate nuclear lamina targeting
    Article Snippet: mouse PRR14 expression vector was obtained from Origene Technologies, Inc., encoding a C-terminal fusion with TurboGFP (cat# MG209414). .. The mouse PRR14 orf was transferred to the N-terminal mGFP vector from OriGene (pCMV6-AN-mGFP, cat# PS100048) using the Origene PrecisionShuttle system. .. For analyses of candidate modular LBDs from human, xenopus, and gecko PRR14, and for expression of the isolated Tantalus domain, gene synth

    Article Title: The Human Protein PRR14 Tethers Heterochromatin to the Nuclear Lamina During Interphase and Mitotic Exit
    Article Snippet: expression vectors were obtained from OriGene Technologies, Inc., encoding C-terminal fusions with TurboGFP (RG208696) or a Myc-DDK-tag (RC208696). .. The PRR14 orf was also transferred to an N-terminal mGFP vector from OriGene (PS100048). .. Deletion mutagenesis was carried out using restriction enzyme sites within the PRR14 orf.

    Article Title: The Human Protein PRR14 Tethers Heterochromatin to the Nuclear Lamina During Interphase and Mitotic Exit
    Article Snippet: Two PRR14 expression vectors were obtained from OriGene Technologies, Inc., encoding C-terminal fusions with TurboGFP (RG208696) or a Myc-DDK-tag (RC208696).Two PRR14 expression vectors were obtained from OriGene Technologies, Inc., encoding C-terminal fusions with TurboGFP (RG208696) or a Myc-DDK-tag (RC208696).. The PRR14 orf was also transferred to an N-terminal mGFP vector from OriGene (PS100048).. Deletion mutagenesis was carried out using restriction enzyme sites within the PRR14 orf.Deletion mutagenesis was carried out using restriction enzyme sites within the PRR14 orf.

    Sequencing:

    Article Title: The PRR14 heterochromatin tether encodes modular domains that mediate and regulate nuclear lamina targeting
    Article Snippet: The mouse PRR14 expression vector was obtained from Origene Technologies, encoding a C-terminal fusion with TurboGFP (cat# MG209414).The mouse PRR14 expression vector was obtained from Origene Technologies, encoding a C-terminal fusion with TurboGFP (cat# MG209414).. The mouse PRR14 ORF was transferred to the N-terminal mGFP vector from OriGene (pCMV6-AN-mGFP, cat# PS100048) using the Origene PrecisionShuttle system.. For analyses of candidate modular LBDs from human, Xenopus , and gecko PRR14, and for expression of the isolated Tantalus domain, gene synthesis was used (Genewiz).For analyses of candidate modular LBDs from human, Xenopus , and gecko PRR14, and for expression of the isolated Tantalus domain, gene synthesis was used (Genewiz).

    Article Title: The PRR14 heterochromatin tether encodes modular domains that mediate and regulate nuclear lamina targeting
    Article Snippet: mouse PRR14 expression vector was obtained from Origene Technologies, Inc., encoding a C-terminal fusion with TurboGFP (cat# MG209414). .. The mouse PRR14 orf was transferred to the N-terminal mGFP vector from OriGene (pCMV6-AN-mGFP, cat# PS100048) using the Origene PrecisionShuttle system. .. For analyses of candidate modular LBDs from human, xenopus, and gecko PRR14, and for expression of the isolated Tantalus domain, gene synth

    Article Title: The Human Protein PRR14 Tethers Heterochromatin to the Nuclear Lamina During Interphase and Mitotic Exit
    Article Snippet: expression vectors were obtained from OriGene Technologies, Inc., encoding C-terminal fusions with TurboGFP (RG208696) or a Myc-DDK-tag (RC208696). .. The PRR14 orf was also transferred to an N-terminal mGFP vector from OriGene (PS100048). .. Deletion mutagenesis was carried out using restriction enzyme sites within the PRR14 orf.

    Article Title: The Human Protein PRR14 Tethers Heterochromatin to the Nuclear Lamina During Interphase and Mitotic Exit
    Article Snippet: Two PRR14 expression vectors were obtained from OriGene Technologies, Inc., encoding C-terminal fusions with TurboGFP (RG208696) or a Myc-DDK-tag (RC208696).Two PRR14 expression vectors were obtained from OriGene Technologies, Inc., encoding C-terminal fusions with TurboGFP (RG208696) or a Myc-DDK-tag (RC208696).. The PRR14 orf was also transferred to an N-terminal mGFP vector from OriGene (PS100048).. Deletion mutagenesis was carried out using restriction enzyme sites within the PRR14 orf.Deletion mutagenesis was carried out using restriction enzyme sites within the PRR14 orf.



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    n terminal mgfp vector  (OriGene)
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    OriGene n terminal mgfp vector
    Identification of a <t>modular</t> <t>PRR14</t> nuclear lamina binding domain. (A) Representative confocal imaging of HeLa cells showing localization of H3K9me3-marked heterochromatin (cyan), GFP-tagged full-length PRR14 (green), and Lamin A/C (red). (B) A schematic model of H3K9me3/HP1 heterochromatin tethering to the nuclear lamina via PRR14. See color scheme in panel C. (C) A schematic representation of the proposed PRR14 modular domain organization. Domains, LAVVL motif and nuclear localization sequences (NLS) are indicated. (D) Representative confocal images of live HeLa cells expressing mCherry–Lamin A transfected with GFP-tagged PRR14 fragments, as indicated (wild type, WT). The <t>N-terminal</t> fragment (1–135) shows localization to heterochromatin, the centrally located (231–282) fragment includes a surrogate SV40 NLS and is identified as a minimal nuclear lamina binding domain (LBD), the Tantalus domain (455–517) fragment localizes throughout the nucleoplasm and cytoplasm and the C-terminal fragment (366–585) localizes in the nucleoplasm. Scale bars: 5 µm. Images in A and D are representative of three experiments and >60 cells.
    N Terminal Mgfp Vector, supplied by OriGene, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/n+terminal+mgfp+vector/pCMV6-AN-mGFP+Mammalian+Expression+Vector/pmc07272351-289-8-12
    Average 91 stars, based on 1 article reviews
    n terminal mgfp vector - by Bioz Stars, 2026-09
    91/100 stars
    		  Buy from Supplier

    Image Search Results


    Identification of a modular PRR14 nuclear lamina binding domain. (A) Representative confocal imaging of HeLa cells showing localization of H3K9me3-marked heterochromatin (cyan), GFP-tagged full-length PRR14 (green), and Lamin A/C (red). (B) A schematic model of H3K9me3/HP1 heterochromatin tethering to the nuclear lamina via PRR14. See color scheme in panel C. (C) A schematic representation of the proposed PRR14 modular domain organization. Domains, LAVVL motif and nuclear localization sequences (NLS) are indicated. (D) Representative confocal images of live HeLa cells expressing mCherry–Lamin A transfected with GFP-tagged PRR14 fragments, as indicated (wild type, WT). The N-terminal fragment (1–135) shows localization to heterochromatin, the centrally located (231–282) fragment includes a surrogate SV40 NLS and is identified as a minimal nuclear lamina binding domain (LBD), the Tantalus domain (455–517) fragment localizes throughout the nucleoplasm and cytoplasm and the C-terminal fragment (366–585) localizes in the nucleoplasm. Scale bars: 5 µm. Images in A and D are representative of three experiments and >60 cells.

    Journal: Journal of Cell Science

    Article Title: The PRR14 heterochromatin tether encodes modular domains that mediate and regulate nuclear lamina targeting

    doi: 10.1242/jcs.240416

    Figure Lengend Snippet: Identification of a modular PRR14 nuclear lamina binding domain. (A) Representative confocal imaging of HeLa cells showing localization of H3K9me3-marked heterochromatin (cyan), GFP-tagged full-length PRR14 (green), and Lamin A/C (red). (B) A schematic model of H3K9me3/HP1 heterochromatin tethering to the nuclear lamina via PRR14. See color scheme in panel C. (C) A schematic representation of the proposed PRR14 modular domain organization. Domains, LAVVL motif and nuclear localization sequences (NLS) are indicated. (D) Representative confocal images of live HeLa cells expressing mCherry–Lamin A transfected with GFP-tagged PRR14 fragments, as indicated (wild type, WT). The N-terminal fragment (1–135) shows localization to heterochromatin, the centrally located (231–282) fragment includes a surrogate SV40 NLS and is identified as a minimal nuclear lamina binding domain (LBD), the Tantalus domain (455–517) fragment localizes throughout the nucleoplasm and cytoplasm and the C-terminal fragment (366–585) localizes in the nucleoplasm. Scale bars: 5 µm. Images in A and D are representative of three experiments and >60 cells.

    Article Snippet: The mouse PRR14 ORF was transferred to the N-terminal mGFP vector from OriGene (pCMV6-AN-mGFP, cat# PS100048) using the Origene PrecisionShuttle system.

    Techniques: Binding Assay, Imaging, Expressing, Transfection